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Fig. 7 | Plant Methods

Fig. 7

From: Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

Fig. 7

Relative quantification of HMGR (a), FPPS (b), TgTPS2 (d) and TgCYP7AE2 (c) expression (qRT-PCR) with three internal controls (actin, tubulin and ef1α) in T. garganica in vitro shoots grown in TIBs. The cultivation was performed in ½MS and ½MS + 400 µM MeJA and the RNA was extracted after 18 days of growth in TIBs. As controls TIBs with MS medium supplemented with BAP (1.5 mg/L) and NAA (0.5 mg/L) were used. The different letters on the bars indicate significant differences based on an ANOVA test followed by a Tukey’s multiple comparison test (p ≤ 0.05). n = 9 bioreactors per treatment

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Plant Methods

ISSN: 1746-4811