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Fig. 4 | Plant Methods

Fig. 4

From: Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

Fig. 4

The effect of reduced nutrient supply; either alone or in combination with MeJA elicitation, on thapsigargin and nortrilobolide content (mg/g DW) in T. garganica in vitro shoots grown 18 days in TIBs. As controls TIBs with MS medium supplemented with BAP (1.5 mg/L) and NAA (0.5 mg/L) were used. Different letters on the bars indicate significant differences based on an ANOVA test followed by a Tukey’s multiple comparison test (p ≤ 0.05). n = 9 bioreactors per treatment. TG = thapsigargin shown in (a), NT = nortrilobolide shown in (b), DW = dry weight

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