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Fig. 3 | Plant Methods

Fig. 3

From: Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures of Thapsia garganica

Fig. 3

The effect of MeJA elicitation on thapsigargin and nortrilobolide content (mg/g DW) in T. garganica in vitro shoots in TIBs. The medium was MS salts and vitamins supplemented with 1.5 mg/L BAP and 0.5 mg/L NAA and different concentrations of MeJA (100, 200 and 400 µM). An asterisk (*) indicates a significant difference between the sample and the control. This is based on an ANOVA test followed by a Tukey’s multiple comparison test (p ≤ 0.05). n = 6–9 samples per treatment.TG = thapsigargin shown in (a), NT = nortrilobolide shown in (b), DW = dry weight

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