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Fig. 1 | Plant Methods

Fig. 1

From: Improving agroinfiltration-based transient gene expression in Nicotiana benthamiana

Fig. 1

Schematic representation of pEAQ-GSN, pSPECIAL and pNEEDS vectors. pEAQ-GSN was assembled by introducing the uidA reporter gene and small synthetic intron (syntron) between the CPMV 5′ and 3′ UTR translation enhancers in pEAQ-HT [31]. pSPECIAL is based on pEAQ-GSN with a downstream expression cassette encoding the truncated CMV 2b (1-94) silencing suppressor protein. pNEEDS is a pBIN-Plus binary vector comprising two expression cassettes encoding the AtBAG4 stress tolerance protein under the transcriptional control of the nos promoter and the TYDV Rep/RepA cell cycle control gene products under the transcriptional control of the truncated CaMV (− 90) promoter. 35SP = Cauliflower mosaic virus 35S promoter; CPMV 5′ UTR = Cowpea mosaic virus RNA‐2 5′UTR; uidA = gene encoding GUS; syntron = synthetic intron; CPMV 3′ UTR = Cowpea mosaic virus RNA‐2 3′UTR; nosT = nopaline synthase terminator from Agrobacterium; TBSV p19 = Tomato bushy stunt virus p19 silencing suppressor gene; 35ST = Cauliflower mosaic virus 35S terminator; CMV 2b (1-94) = Cucumber mosaic virus truncated 2b silencing suppressor gene (amino acids 1-94); nosP = nopaline synthase promoter from Agrobacterium; AtBAG4= Arabidopsis BAG4 gene; ∆35SP = truncated Cauliflower mosaic virus 35S (− 90) promoter; TYDV Rep/RepA = Tobacco yellow dwarf virus Rep/RepA gene encoding both Rep and RepA

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