Skip to main content

Table 3 TMT labelling test for FASP and SDC-FASP protocols

From: Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves

Protocols

Peptides

Proteins

Labelled peptides

Labelling efficiency (%)

Correlation (r)a

R1/R2

R1/R3

R2/R3

FASP

4157

1259

4153

99.9

0.988

0.997

0.986

SDC-FASP

4683

1280

4679

99.9

0.997

0.999

0.999

  1. For each protocol, three replicates of peptides labelled with TMT tags were combined into one sample and then subjected for LC–MS analysis. Peptides labelled with TMT tag were separated using a 60 min LC gradient
  2. aCorrelation between replicates were calculated from the quantified PSM abundance and expressed as r value; R1, R2 and R3 were three replicates of each protocol
Back to article page

Plant Methods

ISSN: 1746-4811