Skip to main content

Table 2 Proteins and peptides quantified using different protocols for protein extraction and digestion

From: Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves

Protocol

Peptide

Protein

Peptide/protein

 

Mean ± SD

ID ≥ 2

Percentage quantified

Mean ± SD

ID ≥ 2

Percentage quantified

FASP

6576 ± 237a

6395

86.4 ± 0.7a

1745 ± 71a

1725

95.2 ± 0.7a

3.8 ± 0.1ab

SDS-ISD

4584 ± 110b

4513

86.1 ± 1.9a

1326 ± 81b

1290

95.5 ± 0.2a

3.5 ± 0.2b

SDC-FASP

6907 ± 219a

6748

86.5 ± 1.0a

1829 ± 98a

1800

94.9 ± 0.3a

3.8 ± 0.1ab

SDC-ISD

4938 ± 424b

4733

86.4 ± 3.5a

1378 ± 51b

1352

95.1 ± 1.4a

3.6 ± 0.2b

OP-ISD

5909 ± 207c

5628

84.5 ± 1,6a

1490 ± 65b

1460

94.4 ± 0.2a

4.0 ± 0.1a

  1. Peptides and proteins were identified and quantified using label-free proteomics
  2. ID ≥ 2, peptides or proteins identified in at least two replicates; percentage quantified, percentage of peptides or proteins quantified from the identified ones in at least two replicates. Different lowercase letters indicate significant difference at 0.05 level. Data are mean ± SD (n = 3)