Skip to main content

Advertisement

Table 2 Proteins and peptides quantified using different protocols for protein extraction and digestion

From: Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves

Protocol Peptide Protein Peptide/protein
  Mean ± SD ID ≥ 2 Percentage quantified Mean ± SD ID ≥ 2 Percentage quantified
FASP 6576 ± 237a 6395 86.4 ± 0.7a 1745 ± 71a 1725 95.2 ± 0.7a 3.8 ± 0.1ab
SDS-ISD 4584 ± 110b 4513 86.1 ± 1.9a 1326 ± 81b 1290 95.5 ± 0.2a 3.5 ± 0.2b
SDC-FASP 6907 ± 219a 6748 86.5 ± 1.0a 1829 ± 98a 1800 94.9 ± 0.3a 3.8 ± 0.1ab
SDC-ISD 4938 ± 424b 4733 86.4 ± 3.5a 1378 ± 51b 1352 95.1 ± 1.4a 3.6 ± 0.2b
OP-ISD 5909 ± 207c 5628 84.5 ± 1,6a 1490 ± 65b 1460 94.4 ± 0.2a 4.0 ± 0.1a
  1. Peptides and proteins were identified and quantified using label-free proteomics
  2. ID ≥ 2, peptides or proteins identified in at least two replicates; percentage quantified, percentage of peptides or proteins quantified from the identified ones in at least two replicates. Different lowercase letters indicate significant difference at 0.05 level. Data are mean ± SD (n = 3)