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Table 1 Timing and difficulty level of different sample preparation protocols for MS-based proteomics

From: Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves

Timing (h) Difficulty levelb Timing (h) Difficulty level Timing (h) Difficulty level Timing (h) Difficulty level Timing (h) Difficulty level
Protein extraction 1.5 1.5 1.5 1.5 1.5
Protein quantification 1 0.5 1 1 1
Clean up at protein level 1.5 9 1 0 0
Reduction and alkylation 0.5 0.5 0.5 0.5 0
Clean up at peptide level 0 0 0.5 0.5 0.5
Digestion 7 6 7 6 6
Total 11.5 17.5 11.5 9.5 9
  1. aprotein quantification can be performed by several methods. The timing is calculated from the method that required least time to be done, i.e. BCA methods for FASP, SDC-FASP, SDC-ISD and OP-IS protocols and Qubit for SDS-ISD
  2. bThe difficulty level reflects the time required, the number of steps to be performed and the complexity of the steps; an open star indicates the easiest step, while the number of filled stars shows the level of difficulty of each step