Procedurea | FASP | SDS-ISD | SDC-FASP | SDC-ISD | OP-ISD | |||||
---|---|---|---|---|---|---|---|---|---|---|
Timing (h) | Difficulty levelb | Timing (h) | Difficulty level | Timing (h) | Difficulty level | Timing (h) | Difficulty level | Timing (h) | Difficulty level | |
Protein extraction | 1.5 | ★★★ | 1.5 | ★★★ | 1.5 | ★★★ | 1.5 | ★★★ | 1.5 | ★★★ |
Protein quantification | 1 | ★ | 0.5 | ★ | 1 | ★ | 1 | ★ | 1 | ★ |
Clean up at protein level | 1.5 | ★★ | 9 | ★★★★ | 1 | ★☆ | 0 | – | 0 | – |
Reduction and alkylation | 0.5 | ☆ | 0.5 | ☆ | 0.5 | ☆ | 0.5 | ☆ | 0 | – |
Clean up at peptide level | 0 | – | 0 | – | 0.5 | ★ | 0.5 | ★ | 0.5 | ★ |
Digestion | 7 | ★☆ | 6 | ☆ | 7 | ★☆ | 6 | ☆ | 6 | ☆ |
Total | 11.5 | ★★★★ ★★★ ☆☆ | 17.5 | ★★★★ ★★★★ ☆☆ | 11.5 | ★★★★ ★★★ ☆☆☆ | 9.5 | ★★★★ ★ ☆☆ | 9 | ★★★★ ★ ☆ |