Fig. 7

In-depth comparison of the FASP and SDC-FASP protocols. Peptides and proteins were identified and quantified using label-free proteomics and in at least two out of three replicates. a and b Venn diagrams of proteins and peptides identified from the FASP and SDC-FASP protocols; c, d, e and f distribution of sequence coverage, molecular weight, isoelectric point (pI) and GRAVY score of proteins specifically identified using the FASP and SDC-FASP protocols; g percentage of specifically identified proteins containing different number (1, between 1 and 6 and > 6) of transmembrane (TM) helices. h Distribution of abundance of peptides (expressed as log₁₀ area) specifically identified using the FASP and SDC-FASP protocols