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Fig. 4 | Plant Methods

Fig. 4

From: Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves

Fig. 4

Abundance distribution of peptides and proteins identified using different protocols for protein extraction and digestion. Peptides and proteins were identified and quantified using label-free proteomics and in at least two out of three replicates. Histograms show the distributions of peptide (a, c, e, g and i) and protein (b, d, f, h and j) abundance among the generated bins of log10 value of MS area. Solid lines are the fitted line of the Gaussian distribution. μ and σ are the mean and standard deviation of the distribution, respectively. Dashed lines indicate the lowest μ of distribution of peptide or protein abundance among different protocols and the percentage of peptide or protein exhibited lower abundance than this value are showed to the left of dashed lines. Protocols of FASP, SDS-ISD, SDC-FASP, SDC-ISD and IP-ISD are shown in Fig. 1

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