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Fig. 4 | Plant Methods

Fig. 4

From: Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

Fig. 4

VIGS assays using roots of infected transgenic 35S::uidA cassava plants. a Mock treatment, b infection with DNA-A + DNA-B, c infection with VIGS-uidA + DNA-B, d infection with VIGS-35S + DNA-B. Adventitious roots from sterile stem cuttings of VIGS-infected cassava were grown in tissue culture and tested for GUS activity after 4 weeks. Intermediate and storage roots of VIGS-infected cassava plants grown in the greenhouse were taken from the soil and tested for GUS activity 6 months post-inoculation. One of the three biological replicates per treatment is shown. Infection rates are presented in Additional file 1: Table S1 A, E and F

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