Fig. 2
From: Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots
Overview of the VIGS assay in cassava. Six week-old cassava plants were injected with a 29G syringe filled with a suspension of mixed Agrobacteria containing either the DNA-A (VIGS vector) or DNA-B plasmids in at least three sites close to axillary meristems. Additional superficial cuts in the lower part of the stem were made using the same syringe to induce the T-DNA release from the Agrobacteria