Fig. 4

Differentially expressed genes determination among three sequencing approaches. a Venn diagram representation of DEG calling in each sequencing approach. b Cross-platform comparison in DEG detection. c Pathway enrichment of each sequencing approach. Black, pathways enriched in all the three approaches; Red, pathways enriched in two approaches; Orange, pathways enriched in one approach. A, alpha-Linolenic acid metabolism; B, Anthocyanin biosynthesis; C, Biosynthesis of secondary metabolites; D, Biosynthesis of unsaturated fatty acids; E, Carotenoid biosynthesis; F, Cutin, suberine and wax biosynthesis; G, Flavonoid biosynthesis; H, Galactose metabolism; I, Glycerolipid metabolism; J, Indole alkaloid biosynthesis; K, Metabolic pathways; L, Other glycan degradation; M, Biosynthesis of secondary metabolites in phenylpropanoid pathway; N, Plant hormone signal transduction; O, Plant-pathogen interaction; P, Starch and sucrose metabolism; Q, Phenylpropanoid biosynthesis; R, Other terpenoid biosynthesis; S, Zeatin biosynthesis; T, MAPK signaling pathway; U, Peroxisome; V, Fatty acid metabolism; W, Pentose and glucuronate interconversions; X, Phenylalanine metabolism