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Table 1 Summary of genomic DNA extractions from a range of species and tissue preparations

From: Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Species

Tissue1

Yield range2 (μg)

Yield mean (μg)

A260/280 absorbance ratio

Sequenced3

White clover (Trifolium repens)

Fresh

1–3

2.5

2.16

GBS

FD

1–6

2.7

2.17

GBS

SG

2–6

4.1

1.97

Western clover (T. occidentale)

Fresh

1.5–13

2.1

2.10

GBS

FD

1.4

1.4

2.09

GBS

SG

1.2

1.2

2.09

Pale clover (T. pallescens)

Fresh

3.5

3.5

2.08

FD

1–6

2.1

1.90

GBS

SG

2.4

2.4

2.11

Red clover (T. pratense)

Fresh

1–3

1.5

1.85

GBS

FD

4.8

4.8

2.12

SG

7.7

7.7

2.11

Sub clover (T. subterraneum)

Fresh

1.9

1.9

1.87

GBS

FD

1–6

1.7

2.00

SG

1.8

1.8

2.16

T. uniflorum

Fresh

2–8

2.3

1.93

GBS

FD

1.2

1.2

2.10

PacBio

SG

1.2

1.2

2.12

Alfalfa/Lucerne (Medicago sativa)

Fresh

1.3

1.3

2.12

FD

2–7

4.0

2.03

GBS

SG

1.7

1.7

2.04

Perennial ryegrass (Lolium perenne)

Fresh

2–5

3.4

2.07

GBS

FD

2.3

2.3

2.04

PacBio

SG

3.5

3.5

2.07

Tall fescue (Festuca arundinacea)

Fresh

1–9

3.8

1.91

GBS

FD

4.4

4.4

2.07

SG

4.5

4.5

2.07

Rye (Secale cereale)

Fresh

2–7

3.8

1.84

GBS

FD

SG

Apple (Malus pumila)

Fresh

1–2

1.2

2.13

FD

1–2

1.1

1.99

SG

1–2

1.4

2.14

Rice (Oryza sativa)

Fresh

2–4

3.2

2.06

FD

1.4

1.4

2.11

SG

2.3

2.3

2.10

 

Thale cress (Arabidopsis thaliana)

Fresh

0.6

0.6

2.15

FD

0.5

0.5

2.13

SG

0.2

0.2

2.21

  1. 1FD freeze-dried; SD silica gel-dried
  2. 2A single number indicates a single extraction was quantified from that species and tissue
  3. 3GBS used routinely for genotyping-by-sequencing and passes FastQC [9] sequencing quality parameters. PacBio used to generate quality PacBio long-read sequencing data
  4. Grasses, cereal and rice samples were pseudostem, whereas all other plant samples were leaf lamina