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Table 1 Summary of genomic DNA extractions from a range of species and tissue preparations

From: Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Species Tissue1 Yield range2 (μg) Yield mean (μg) A260/280 absorbance ratio Sequenced3
White clover (Trifolium repens) Fresh 1–3 2.5 2.16 GBS
FD 1–6 2.7 2.17 GBS
SG 2–6 4.1 1.97
Western clover (T. occidentale) Fresh 1.5–13 2.1 2.10 GBS
FD 1.4 1.4 2.09 GBS
SG 1.2 1.2 2.09
Pale clover (T. pallescens) Fresh 3.5 3.5 2.08
FD 1–6 2.1 1.90 GBS
SG 2.4 2.4 2.11
Red clover (T. pratense) Fresh 1–3 1.5 1.85 GBS
FD 4.8 4.8 2.12
SG 7.7 7.7 2.11
Sub clover (T. subterraneum) Fresh 1.9 1.9 1.87 GBS
FD 1–6 1.7 2.00
SG 1.8 1.8 2.16
T. uniflorum Fresh 2–8 2.3 1.93 GBS
FD 1.2 1.2 2.10 PacBio
SG 1.2 1.2 2.12
Alfalfa/Lucerne (Medicago sativa) Fresh 1.3 1.3 2.12
FD 2–7 4.0 2.03 GBS
SG 1.7 1.7 2.04
Perennial ryegrass (Lolium perenne) Fresh 2–5 3.4 2.07 GBS
FD 2.3 2.3 2.04 PacBio
SG 3.5 3.5 2.07
Tall fescue (Festuca arundinacea) Fresh 1–9 3.8 1.91 GBS
FD 4.4 4.4 2.07
SG 4.5 4.5 2.07
Rye (Secale cereale) Fresh 2–7 3.8 1.84 GBS
FD
SG
Apple (Malus pumila) Fresh 1–2 1.2 2.13
FD 1–2 1.1 1.99
SG 1–2 1.4 2.14
Rice (Oryza sativa) Fresh 2–4 3.2 2.06
FD 1.4 1.4 2.11
SG 2.3 2.3 2.10  
Thale cress (Arabidopsis thaliana) Fresh 0.6 0.6 2.15
FD 0.5 0.5 2.13
SG 0.2 0.2 2.21
  1. 1FD freeze-dried; SD silica gel-dried
  2. 2A single number indicates a single extraction was quantified from that species and tissue
  3. 3GBS used routinely for genotyping-by-sequencing and passes FastQC [9] sequencing quality parameters. PacBio used to generate quality PacBio long-read sequencing data
  4. Grasses, cereal and rice samples were pseudostem, whereas all other plant samples were leaf lamina