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Fig. 3 | Plant Methods

Fig. 3

From: Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Fig. 3

A typical DNA extraction from freeze-dried and fresh tissue using the 96-well plate method. Genomic DNA was extracted from 96 individuals of a Trifolium repens (white clover; freeze-dried leaf) and b Lolium perenne (perennial ryegrass; fresh pseudostem) using the 96-well plate protocols, and resolved and visualised by electrophoresis on an agarose lithium borate buffer (0.8% w/v) gel containing 25 μg ethidium bromide. The samples were flanked at either end by 1 Kb Plus size ladders (www.thermofisher.com). One T. repens individual had a poor DNA yield (top row, ninth lane from the right) and was subsequently re-extracted using the Individual Tube freeze-dried tissue protocol. These DNA samples have been used for developing genotyping-by-sequencing libraries and yielded high-quality sequence data

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