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Fig. 2 | Plant Methods

Fig. 2

From: Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Fig. 2

Genomic DNA (gDNA) extracted from fresh, freeze-dried, and silica gel-dried plant tissue. Samples of gDNA extracted from a clover leaf tissue or grass pseudostem b rice pseudostem and c Arabidopsis thaliana leaf tissue using the high-throughput protocol were resolved and visualised by electrophoresis in an agarose lithium borate buffer (0.8% w/v) gel containing 25 μg ethidium bromide. F fresh, Fd freeze-dried, Sd silica gel-dried; Tr Trifolium repens (white clover); Tp T. pratense (red clover); Lp Lolium perenne (perennial ryegrass); Fa Festuca arundinacea (tall fescue); Os Oryza sativa (rice); At Arabidopsis thaliana; λ λ DNA standard. The samples were flanked by 1 Kb Plus size ladders (www.thermofisher.com)

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Plant Methods

ISSN: 1746-4811