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Fig. 1 | Plant Methods

Fig. 1

From: Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing

Fig. 1

Genomic DNA (gDNA) extracted and restriction endonuclease-digested from forage legumes and grasses, and horticultural species. Samples of gDNA a extracted from freeze-dried leaf tissue or grass pseudostem using the high-throughput protocol and b digested with HindIII restriction endonuclease were resolved and visualised by electrophoresis in an agarose lithium borate buffer (0.8% w/v) gel containing 25 μg ethidium bromide. The samples were, in order: (1) 1 kb Plus size ladder (www.thermofisher.com); (2) Trifolium repens (white clover); (3) T. occidentale (western clover); (4) T. pallescens (pale clover); (5) T. pratense (red clover); (6) T. subterraneum (subterranean clover); (7) T. uniflorum (8) Medicago sativa (alfalfa/lucerne); (9) Lolium perenne (perennial ryegrass); (10) Festuca arundinacea (tall fescue); (11) Secale cereale (rye); (12) Malus pumila (apple); (13) λ DNA standard, and (14) 1 kb Plus size ladder. The λ DNA standard was digested with HindIII in (B)

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