Dehiscence method: a seed-saving, quick and simple viability assessment in rice

Table 2 Abbreviation and description of seed treatments

JG	Under uncontrolled ageing treatment (UA, without priming/imbibition) till Jul. 2015 and was then stored at − 18 °C until Nov. 2017. NPB14-UA1 and UA2 were NPB14 seeds of different viability after UA
93-11
NPB14
NPB14	NPB14-NA were naturally aged since harvest for 3 years and germination percentage was 28% NPB14-NA-HP were hydroprimed NPB14-NA
NPB16	N16-1–8d	NPB16 under artificial accelerated ageing (AAA) for 1–8 d
	Post-ageing Priming	NPB16-5d-Spd and NPB16-5d-HP were NPB16-5d primed with 1 mM spermidine and distill water respectively; NPB16-HP were NPB16 primed with distill water without AAA
	Post-Dehiscence Ageing	AAA and natural ageing after recollecting and dehydrating dehiscent seeds previously in distill water or 1 mM hydrogen peroxide

Germination for 24 h is also the incubation process of priming where seeds were redried before protrusion

ISSN: 1746-4811