Fig. 5

TTC Staining of control and low-vigorous NPB14 seeds. a In the control (GP ≥ 95%) seeds were judged viable for they are completely stained, while none of them were dehiscent. Embryo a1 and a2: R, radicle; P, plumule/shoot. All radicles and shoots were entirely stained and none of the shoot protruded out of the top of the embryo. b In GP = 43% treatment seeds were judged viable for protrusion of shoots but most of them could not be labelled viable by TTC for failure to stain the radicle area (blank area with the frames). Embryo b1’s major radicle was stained but the tip area not. b1’s and b3’s shoot got broken during the dissecting but were still apparent to protrude as b2 and b4