Factors affecting the efficiency of Rhizobium rhizogenes root transformation of the root parasitic plant Triphysaria versicolor and its host Arabidopsis thaliana

Table 5 Effect of temperature and co-incubation period on Arabidopsis root transformation

Duration in co-transformation plate (days)	Growth temperature (°C)	Transformation efficiency (Mean ± SD)
3	25	72.05 ± 5.7^a
3	16	14.86 ± 3.4^b
6	25	32.82 ± 9.2^a
6	16	6.40 ± 0.9^b
9	25	13.56 ± 4.4^a
9	16	3.29 ± 1.5^b
12	25	2.50 ± 2.4^a
12	16	0.49 ± 0.3^a
Duration in 16 °C Pr > F 0.0001
Growth temperature (°C) Pr > F 0.0001
Duration in 16 °C * Growth temperature(°C) Pr > F 0.0001

Each treatment consisted of five plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 09). Significantly different transformation efficiencies are represented by different letters

ISSN: 1746-4811