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Table 4 Effects of temperature on transformation and subsequent growth of Triphysaria roots

From: Factors affecting the efficiency of Rhizobium rhizogenes root transformation of the root parasitic plant Triphysaria versicolor and its host Arabidopsis thaliana

Measurement

Time at 16 °C

Statistics

2 weeks at 16 °C + 6 weeks in 25 °C

8 weeks at 16 °C

Transformation efficiency (%)

28.25 ± 13.9b

62.36 ± 4.0a

Pr > |t| 0.0149

Total number of roots

13.23 ± 09a

6.87 ± 0.7b

Pr > |t| 0.0006

Number of YFP roots

1.15 ± 0.0a

1.52 ± 0.2a

Pr > |t| 0.1355

% YFP roots

9.81 ± 0.8b

24.58 ± 2.9a

Pr > |t| 0.0011

Length of YFP roots (cm)

3.30 ± 0.1a

2.31 ± 0.1b

Pr > |t| 0.0005

  1. Each treatment consisted of three plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 09). Mean transformation efficiency, of a measurement, represented by different letters are significantly different at the P value given
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Plant Methods

ISSN: 1746-4811