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Table 3 Effects of culture medium on Triphysaria transformation and subsequent root growth

From: Factors affecting the efficiency of Rhizobium rhizogenes root transformation of the root parasitic plant Triphysaria versicolor and its host Arabidopsis thaliana

Measurement

Medium

Statistics

0.25X Hoagland

MS

% of YFP calli

73.26 ± 3.1a

75.21 ± 4.1a

Pr > |t| 0.5482

Transformation efficiency %

64.66 ± 4.4a

17.86 ± 9.3b

Pr > |t| 0.0014

Total number of roots

7.50 ± 0.3a

3.20 ± 0.1b

Pr > |t| < 0.0001

Number of YFP roots

2.00 ± 0.3a

0.25 ± 0.1b

Pr > |t| 0.0005

% of YFP roots

29.08 ± 4.8a

5.60 ± 1.6b

Pr > |t| 0.0013

Root length

2.67 ± 0.3a

1.91 ± 0.1b

Pr > |t| 0.0117

  1. Each treatment consisted of three plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 09). Mean transformation efficiency, of a measurement, represented by different letters are significantly different at the P value given
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Plant Methods

ISSN: 1746-4811