Factors affecting the efficiency of Rhizobium rhizogenes root transformation of the root parasitic plant Triphysaria versicolor and its host Arabidopsis thaliana

Table 2 Effects of biological and environmental parameters on Triphysaria root transformation

Parameter tested	Levels	Transformation efficiency	Statistics
Sugar content in the medium	0%	2.26 ± 0.8^c	Pr > F < 0.0001
	0.5%	11.74 ± 5.0^bc
	1%	20.84 ± 5.3^b
	3%	73.93 ± 9.0^a
	4%	74.07 ± 6.0^a
Age of the seedling	5 days	67.91 ± 4.1^a	Pr > F < 0.0013
	10 days	54.18 ± 2.4^b
	15 days	37.31 ± 8.1^c
Acetosyringone	0	3.25 ± 0.9^c	Pr > F < 0.0001
	200 uM	21.74 ± 4.5^b	n = 366–376
	400 uM	65.03 ± 6.4^a
Days kept in the co-incubation plate	7 days	33.21 ± 8.5^c	Pr > F < 0.0008
	14 days	68.33 ± 4.2^a
	21 days	61.26 ± 4.1^a
Explant type	Hypocotyl	67.20 ± 2.8^a	Pr > \|t\| < 0.0001
Explant type	Root tip	0.76 ± 0.8^b	Pr > \|t\| < 0.0001
Illumination during co-incubation	Light	67.53 ± 6.7^a	Pr > \|t\| < 0.0001
Illumination during co-incubation	Dark	12.46 ± 2.6^b	Pr > \|t\| < 0.0001

Each treatment consisted of three plates (each plate is a technical replicate) and the experiments were conducted three times (biological replicates). The efficiency values represent the average ± SD (n = 09). Mean transformation efficiency, of a parameter tested, represented by different letters are significantly different at the P value given

ISSN: 1746-4811