Fig. 3

Confirmation of R. rhizogenes Ri plasmid integration in YFP expressing T. versicolor and A. thaliana roots. Primers for rolB, rolC, virD2, TvQR1 and AtActin were used in PCR reactions using cDNA from transgenic (T) or non-transformed (Wt) roots. MSU is genomic DNA from MSU440 and M is the 1 Kb+ DNA ladder. The cDNA from wild type roots and genomic DNA from R. rhizogenes MSU440 were used as negative and positive controls. T. versicolor TvQR1 gene and A. thaliana Actin gene were used as a positive control for amplification plant cDNA. The rolB and rolC genes were amplified from hairy root cDNA. The virD2, an R. rhizogenes gene present on the Ri plasmid but not inserted into the plant genome, was amplified from YFP expressing roots