Fig. 5From: In vivo monitoring of plant small GTPase activation using a Förster resonance energy transfer biosensorValidating the Raichu-OsRac1 FRET sensor. a, b Emission ratio images of rice protoplasts expressing Raichu-OsRac1 mutants. Rice protoplasts were transfected with constructs expressing Raichu-OsRac1 WT, mutants and the PRONE domain of OsRacGEF1. Double asterisks indicate significant differences from the data for WT-OsRac1 or control GUS enzyme (P < 0.01). Error bars indicate SE (n > 30)Back to article page