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Fig. 5 | Plant Methods

Fig. 5

From: In vivo monitoring of plant small GTPase activation using a Förster resonance energy transfer biosensor

Fig. 5

Validating the Raichu-OsRac1 FRET sensor. a, b Emission ratio images of rice protoplasts expressing Raichu-OsRac1 mutants. Rice protoplasts were transfected with constructs expressing Raichu-OsRac1 WT, mutants and the PRONE domain of OsRacGEF1. Double asterisks indicate significant differences from the data for WT-OsRac1 or control GUS enzyme (P < 0.01). Error bars indicate SE (n > 30)

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