Fig. 6

Subcellular localization of GFP-tagged GhGPX1 and GhGPX8 in G. hirsutum. a Transient expression of control vector pro35S-GFP. b Transient expression of the Pro35S-GhGPX1-GFP construct resulted in strong GFP fluorescence in the chloroplasts of epidermal and guard cells detected by confocal microscopy. Pictures in the bottom line show a close up of a guard cell. c Confocal microscopy of plants transiently expressing the Pro35S-GhGPX8-GFP construct shows fluorescence in the cytosol of epidermal and root cells. White bars, 5 μm; red bars, 10 μm; yellow bars, 100 μm. At least 7 different seedlings from three independent biological and technical replicates were used for each experiment with similar results