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Fig. 3 | Plant Methods

Fig. 3

From: A transient transformation system for gene characterization in upland cotton (Gossypium hirsutum)

Fig. 3

Expression patterns of GhGPX1 promoter-GUS constructs in EHA105-inoculated G. hirsutum plants under different transformation condition. GUS staining (a, c, e, g) and relative GUS activity (b, d, f, h) of plants transiently expressing GhGPX1 promoter constructs using Agrobacterium EHA105; a, b, effect of different Agrobacterium concentrations; c, d, GhGPX1 expression levels at different times after transformation; e, f, effect of different acetosyringone (AS) concentrations; g, h, effect of different types and concentrations of surfactants. At least three independent seedlings were used for GUS staining. GUS activity values are the means of approximately 5 seedlings. The GUS activity of plants transformed with an Agrobacterium density of OD600 = 0.5 (a), infected for 3 days (b), using an AS concentration of 80 μM (c) and no surfactants (d) were given an arbitrary value of 1. For each sample of GUS activity assay, at least three different sections of a single cotyledon from one seedling were processed separately as three technical replicates, and three different seedlings were used to make three biological replicates. Asterisks indicate significant differences from WT at *p < 0.05 and **p < 0.01 (two tailed Students t-test)

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