Skip to main content

Advertisement

Table 1 Primary screening with different methods used for root transformation. Efficiency of two different methods used for root transformation of chickpea (Cicer arietinum cultivar Annigeri) seedlings

From: Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Method used for hairy root transformation by A. rhizogenes K599 Total number of explants inoculated Explants with antibiotic selection positive roots (%)
Infection through cotyledonary node injection in intact seedlings** 50 38.66 (± 3.05)b*
Infection at the cut end of the hypocotyl** 50 73.33 (± 1.15)a
  1. Each value represents the mean of three independent experiments with standard deviation (SD). Approximately 50 seedlings were examined for each individual experiment
  2. Values in the same column followed by different letters showed significant difference among different transformation methods (Fisher’s LSD test, P < 0.05)
  3. **Co-cultivation was done at 22 °C for 4 days