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Table 1 Primary screening with different methods used for root transformation. Efficiency of two different methods used for root transformation of chickpea (Cicer arietinum cultivar Annigeri) seedlings

From: Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Method used for hairy root transformation by A. rhizogenes K599

Total number of explants inoculated

Explants with antibiotic selection positive roots (%)

Infection through cotyledonary node injection in intact seedlings**

50

38.66 (± 3.05)b*

Infection at the cut end of the hypocotyl**

50

73.33 (± 1.15)a

  1. Each value represents the mean of three independent experiments with standard deviation (SD). Approximately 50 seedlings were examined for each individual experiment
  2. ∗Values in the same column followed by different letters showed significant difference among different transformation methods (Fisher’s LSD test, P < 0.05)
  3. **Co-cultivation was done at 22 Â°C for 4 days