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Fig. 3 | Plant Methods

Fig. 3

From: Genotype-independent Agrobacterium rhizogenes-mediated root transformation of chickpea: a rapid and efficient method for reverse genetics studies

Fig. 3

Illustrations depicting the main steps for the root transformation of chickpea cultivar Annigeri. a Plants grown for 5 days in MS medium, b germinated chickpea seedling, c, d sectioning the root near hypocotyl region, e immersing the cut end into A. rhizogenes suspension, f explant transferred in co-cultivation medium, g explant transferred to selection medium after co-cultivation, h, i transformed roots grown in the selection medium after 3 and 7 days respectively. After 7 days, plantlets were transferred to pots, j plants grown in pot after 15 days, k completely grown hairy root system in chickpea. Scale bar represents 10 mm

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