Fig. 3
Illustrations depicting the main steps for the root transformation of chickpea cultivar Annigeri. a Plants grown for 5Â days in MS medium, b germinated chickpea seedling, c, d sectioning the root near hypocotyl region, e immersing the cut end into A. rhizogenes suspension, f explant transferred in co-cultivation medium, g explant transferred to selection medium after co-cultivation, h, i transformed roots grown in the selection medium after 3 and 7Â days respectively. After 7Â days, plantlets were transferred to pots, j plants grown in pot after 15Â days, k completely grown hairy root system in chickpea. Scale bar represents 10Â mm