Skip to main content

Table 4 Micrograft success and virus transmission in micrografts of ASGV-infected shoots regenerated from cryopreservation and shoot tip culture in ‘Gala’ apple

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment

Success of micrografts (%)

Virus transmission (%) after different time points (day) of micrografting

3

7

14

21

Drop-vitri

95 ± 5a

0 (0/20)

30 (6/20)

70 (14/20)

100 (20/20)

Encap-vitri

95 ± 5a

0 (0/20)

30 (6/20)

60 (12/20)

100 (20/20)

Shoot tip culture

100 ± 5a

0 (0/20)

30 (6/20)

70 (14/20)

100 (20/20)

  1. Data of success of micrografts are recorded after 21 days of micrografting. Results are presented as mean ± SE and with different letters indicating significances at P < 0.05. Numbers in parenthesis are number of samples showing positive response to RT-PCR/total number of samples tested
  2. Drop-vitri droplet-vitrification, Encap-vitri encapsulation-vitrification