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Table 4 Micrograft success and virus transmission in micrografts of ASGV-infected shoots regenerated from cryopreservation and shoot tip culture in ‘Gala’ apple

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment Success of micrografts (%) Virus transmission (%) after different time points (day) of micrografting
3 7 14 21
Drop-vitri 95 ± 5a 0 (0/20) 30 (6/20) 70 (14/20) 100 (20/20)
Encap-vitri 95 ± 5a 0 (0/20) 30 (6/20) 60 (12/20) 100 (20/20)
Shoot tip culture 100 ± 5a 0 (0/20) 30 (6/20) 70 (14/20) 100 (20/20)
  1. Data of success of micrografts are recorded after 21 days of micrografting. Results are presented as mean ± SE and with different letters indicating significances at P < 0.05. Numbers in parenthesis are number of samples showing positive response to RT-PCR/total number of samples tested
  2. Drop-vitri droplet-vitrification, Encap-vitri encapsulation-vitrification