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Table 3 Relative mRNA expressions levels (Cq values) of ASGV by qRT-PCR in the virus-infected shoots regenerated from cryopreservation of the virus-infected shoots of ‘Gala’ apple after different subculture times

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment

Subculture times after shoot regrowth

1*

2

4

ASGV

EF-1α

ASGV

EF-1α

ASGV

EF-1α

Drop-vitri

32.3 ± 0.4a

15.2 ± 0.8x

28.1 ± 0.6a

15.7 ± 0.4x

21.4 ± 0.5a

15.5 ± 0.3x

Encap-dehy

31.6 ± 0.6a

15.8 ± 0.7x

29.1 ± 0.3a

15.1 ± 0.4x

21.8 ± 0.6a

16.1 ± 0.4x

Shoot tip culture

26.2 ± 0.6b

16.3 ± .04x

22.5 ± 0.6b

15.2 ± 0.5x

20.2 ± 0.5a

15.1 ± 0.5x

  1. Data are expressed in mean ± SE and with different letters indicating significant differences at P < 0.05. EF-1α gene was used as reference gene
  2. Drop-vitri droplet-vitrification, Encap-dehy encapsulation-dehydration
  3. *Numbers 1, 2 and 4 represent 1, 2 and 4 times of subculture, respectively
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Plant Methods

ISSN: 1746-4811