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Table 3 Relative mRNA expressions levels (Cq values) of ASGV by qRT-PCR in the virus-infected shoots regenerated from cryopreservation of the virus-infected shoots of ‘Gala’ apple after different subculture times

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment Subculture times after shoot regrowth
1* 2 4
ASGV EF- ASGV EF- ASGV EF-
Drop-vitri 32.3 ± 0.4a 15.2 ± 0.8x 28.1 ± 0.6a 15.7 ± 0.4x 21.4 ± 0.5a 15.5 ± 0.3x
Encap-dehy 31.6 ± 0.6a 15.8 ± 0.7x 29.1 ± 0.3a 15.1 ± 0.4x 21.8 ± 0.6a 16.1 ± 0.4x
Shoot tip culture 26.2 ± 0.6b 16.3 ± .04x 22.5 ± 0.6b 15.2 ± 0.5x 20.2 ± 0.5a 15.1 ± 0.5x
  1. Data are expressed in mean ± SE and with different letters indicating significant differences at P < 0.05. EF- gene was used as reference gene
  2. Drop-vitri droplet-vitrification, Encap-dehy encapsulation-dehydration
  3. *Numbers 1, 2 and 4 represent 1, 2 and 4 times of subculture, respectively