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Table 2 Shoot proliferation of ASGV-infected apple ‘Gala’ shoots regenerated from cryopreservation and shoot tip culture in different times of subculture

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment

No. of shoots per explant

Mean shoot length (mm)

1*

2

4

1

2

4

Drop-vitri

0a

1.1 ± 0.2a

2.5 ± 0.4a

11.8 ± 0.5a

18.8 ± 1.1a

26.8 ± 2.4a

Encap-dehy

0a

1.1 ± 0.2a

2.5 ± 0.5a

12.8 ± 0.7a

19.8 ± 1.5a

26.8 ± 2.4a

Shoot tip culture

2.6 ± 0.5b

2.8 ± 0.4b

2.6 ± 0.5a

27.2 ± 2.1b

28.1 ± 2.2b

27.3 ± 2.2a

  1. Data are presented as mean ± SE and with different letter in the same column indicating significant differences at P < 0.05
  2. Drop-vitri droplet-vitrification, Encap-dehy encapsulation-dehydration
  3. *Numbers 1, 2 and 4 represent 1, 2 and 4 times of subculture, respectively