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Table 2 Shoot proliferation of ASGV-infected apple ‘Gala’ shoots regenerated from cryopreservation and shoot tip culture in different times of subculture

From: Cryopreservation of virus: a novel biotechnology for long-term preservation of virus in shoot tips

Treatment No. of shoots per explant Mean shoot length (mm)
1* 2 4 1 2 4
Drop-vitri 0a 1.1 ± 0.2a 2.5 ± 0.4a 11.8 ± 0.5a 18.8 ± 1.1a 26.8 ± 2.4a
Encap-dehy 0a 1.1 ± 0.2a 2.5 ± 0.5a 12.8 ± 0.7a 19.8 ± 1.5a 26.8 ± 2.4a
Shoot tip culture 2.6 ± 0.5b 2.8 ± 0.4b 2.6 ± 0.5a 27.2 ± 2.1b 28.1 ± 2.2b 27.3 ± 2.2a
  1. Data are presented as mean ± SE and with different letter in the same column indicating significant differences at P < 0.05
  2. Drop-vitri droplet-vitrification, Encap-dehy encapsulation-dehydration
  3. *Numbers 1, 2 and 4 represent 1, 2 and 4 times of subculture, respectively