Fig. 2
From: A simple and cost-effective method for screening of CRISPR/Cas9-induced homozygous/biallelic mutants
PCR amplification of some synthesized mutated templates with different primer combinations. a WT sequence and PCR primers used in the amplification reactions. The CRISPR/Cas9 target sequence (red) and PAM (blue) are shown in the WT sequence. b Gel electrophoresis was used to analyze the PCR products for different templates and primer combinations. Red asterisks denote the position of the nucleotide deletions in the templates