Skip to main content

Table 1 Optimized conditions for SYS quantification and method validation

From: Accurate and easy method for systemin quantification and examining metabolic changes under different endogenous levels

(a)

     

Compound

Weighted calibration equation

R2

Precision (%RSD)

Intra-day/Inter-day

%RE

% Efficiency

Systemin

y = 1.11989x − 0.04709

0.9945

2.12/2.15

97.73

99.23

(b)

    

Compound

Sequence

MRM (m/z)

Cone voltage (V)

Collision energy (eV)

SYSTEMIN

AVQSKPPSKRDPPKMQTD

503.5 > 143.1

35.0

14.0

  

503.5 > 608.2

35.0

14.0

  

503.5 > 614.3

35.0

14.0

SYSTEMIN*

A{Val(13C5, 15 N)} AVQSKPPSKRDPPKMQTD

505.0 > 148.1

35.0

14.0

  1. (a) Parameters of the calibration curve for SYS and parameters used for method validation. RSD relative standard deviation; RE recovery. LOD (0.011 µg ml−1) and LOQ (0.033 µg ml−1) was calculated by signal to ratio noise of 3 and 10 respectively. A weighting factor of 1/x2 was applied to the curve
  2. (b) Optimized MS/MS conditions for the analysis of SYS. Optimal conditions were selected by infusing the standards of SYS and SYS* to determinate appropriate cone and collision energies to obtain the characteristic transitions for each peptide
Back to article page

Plant Methods

ISSN: 1746-4811