Fig. 6
From: Bioorthogonal click chemistry for fluorescence imaging of choline phospholipids in plants
Dual visualization of choline phospholipids and GFP marker lines. Arabidopsis seeds were germinated and seedlings were harvested for click chemistry after 7 days on agar media containing 200 µM propargylcholine. a–d Alexa Fluor 594 azide (red) indicates propargyl-PC, a′–d′ GFP (or YFP) (green) marks a specified intracellular compartment, and a″–d″ merged images indicate overlapping expression as yellow or orange, as imaged by confocal laser scanning microscopy. a Hypocotyl epidermal cell shows localization of a mitochondrial marker in comparison to propargyl-PC; b–d root epidermal cells indicate localization of markers for b endoplasmic reticulum (ER), c Golgi apparatus, and d tonoplast. Co-localization was quantified using the Pearson correlation coefficient (PCC) (see Additional file 3: Table S1)