Fig. 2

Propargylcholine labeling does not adversely affect plant growth. a–e Arabidopsis plants were germinated and grown on agar medium either with 0 µM propargylcholine (negative control), or with a–d 250 μM or e 200 μM propargylcholine. a Root lengths were compared for 7-day-old seedlings grown in the presence or absence of propargylcholine. b Percent seed germination was not significantly different on media containing propargylcholine versus the control. Error bars indicate the standard deviation (SD) for the mean of three replicates (n ≥ 27; two-tailed t-test, P = 0.38), d Growth on 250 μM propargylcholine did not affect root length. Error bars denote the SD for the mean of three replicates (n ≥ 9; two-tailed t-test, P = 0.64), d Fourteen-day-old seedlings grown on propargylcholine exhibited similar emergence of cotyledons and first leaves as the control. e Six-week-old plants grown in Magenta boxes display comparable growth physiology at the reproductive phase of development