Fig. 4
Analysis of the effect of systemic salt stress on the meiotic crossover frequency of F1 Col3-4/20 (♂) × Ler-0 (♀) hybrids. a Photos of F1 hybrids 8 days after treatments (50 days post germination) with NaCl solutions of the indicated concentrations in demineralized water. b Frequency of meiotic crossovers between the GFP and RFP fluorescence markers at the top of chromosome 3 detected in the F2 seeds. Black dots represent the crossover frequencies of individual F1 hybrids (n ≥ 8 per treatment, except for 275 mM NaCl in which case n = 4). Crossover frequencies were normalized to the control treatment with 0 mM NaCl to combine data sets. Grey dots represent the average crossover frequency. F1 hybrids of which F2 KASP genotyping data are presented in panel C are indicated by black arrows. c Genome-wide KASP genotyping analysis on the F2 seedling progeny of F1 hybrids treated with either 0 mM NaCl or 300 mM NaCl. Plotted is the recombination rate per Mbp on the physical map based on 39 SNP marker sets