Comparison of six different methods to calculate cell densities

Table 4 Agreement between methods using Lin’s concordance correlation coefficient

Comparison	Lin’s concordance correlation coefficient
	Microspore cultures		Fluorosphere suspensions
	Days 3	Days 18	Undiluted	1:2 dilution	1:10 dilution
Neubauer
Flow cytometry + PI	0.75	0.52	0.01	0.01	0.63
Flow cytometry + SSC	0.72	0.49	0.04	0.01	0.63
Automated counter	0.35	0.45	0.00	0.22	0.07
Field counting	− 0.01	0.52	− 0.01	0.21	− 0.04
Flow cytometry + PI
Flow cytometry + SSC	1.00	1.00	0.69	1.00	1.00
Automated counter	0.40	0.64	0.23	− 0.20	0.22
Field counting	− 0.01	0.52	0.24	− 0.14	0.10
Flow cytometry + SSC
Automated counter	0.41	0.62	0.20	− 0.22	0.22
Field counting	0.01	0.51	0.21	− 0.16	0.10
Automated counter
Field counting	0.48	0.88	0.99	0.99	0.98

See text for further details. High, moderate and low agreement values are represented in bolditalic, regular and italics characters, respectively

ISSN: 1746-4811