Skip to main content

Table 2 Mean and standard deviation (first row), median and 1st–3rd quartile (second row), and percentage of deviation from the initial value and coefficient of variation expressed in percentage (third row) of each counting method and dilution of fluorosphere suspensions

From: Comparison of six different methods to calculate cell densities

Method

1/10 dilution

1/2 dilution

Undiluted

Neubauer

87.2 (15.5)

423.3 (84.3)

797.9 (67.8)

92.7 (90.0–96.3)

432.3 (412.7–440.0)

795.0 (769.7–854.7)

15.3/17.8%

17.8/29.9%

22.5/8.5%

Flow cytometry + PI

73.5 (13.8)

287.2 (40.4)

554.6 (128.3)

77.3 (69.8–80.7)

292.7 (283.4–317.6)

559.0 (477.4–599.4)

28.7/18.8%

44.2/14.1%

46.2/23.1%

Flow cytometry + SSC

73.4 (13.8)

289.5 (42.7)

513.7 (166.1)

77.2 (69.7–80.6)

293.3 (284.8–318.6)

491.2 (411.1–602.1)

28.7/18.8%

43.8/14.7%

50.1/32.3%

Automated counter

74.1 (28.4)

361.8 (92.6)

278.6 (96.6)

76.1 (60.7–82.4)

369.5 (291.7–392.1)

299.1 (184.8–318.6)

28.1/38.3%

29.7/25.6%

73.0/34.7%

Field counting

77.2 (29.2)

359.2 (91.9)

286.2 (95.1)

79.9 (66.4–86.9)

343.9 (295.2–397.1)

315.1 (192.2–318.6)

25.0/37.8%

30.3/25.6%

72.2/33.2%

Real value

103.0

515.0

1030.0

  1. Values are expressed in thousands of fluorospheres/ml