From: Comparison of six different methods to calculate cell densities
Method | 1/10 dilution | 1/2 dilution | Undiluted |
---|---|---|---|
Neubauer | 87.2 (15.5) | 423.3 (84.3) | 797.9 (67.8) |
92.7 (90.0–96.3) | 432.3 (412.7–440.0) | 795.0 (769.7–854.7) | |
15.3/17.8% | 17.8/29.9% | 22.5/8.5% | |
Flow cytometry + PI | 73.5 (13.8) | 287.2 (40.4) | 554.6 (128.3) |
77.3 (69.8–80.7) | 292.7 (283.4–317.6) | 559.0 (477.4–599.4) | |
28.7/18.8% | 44.2/14.1% | 46.2/23.1% | |
Flow cytometry + SSC | 73.4 (13.8) | 289.5 (42.7) | 513.7 (166.1) |
77.2 (69.7–80.6) | 293.3 (284.8–318.6) | 491.2 (411.1–602.1) | |
28.7/18.8% | 43.8/14.7% | 50.1/32.3% | |
Automated counter | 74.1 (28.4) | 361.8 (92.6) | 278.6 (96.6) |
76.1 (60.7–82.4) | 369.5 (291.7–392.1) | 299.1 (184.8–318.6) | |
28.1/38.3% | 29.7/25.6% | 73.0/34.7% | |
Field counting | 77.2 (29.2) | 359.2 (91.9) | 286.2 (95.1) |
79.9 (66.4–86.9) | 343.9 (295.2–397.1) | 315.1 (192.2–318.6) | |
25.0/37.8% | 30.3/25.6% | 72.2/33.2% | |
Real value | 103.0 | 515.0 | 1030.0 |