Table 1 Mean and standard deviation (first row), median and 1st–3rd quartile (second row), and percentage of deviation from the initial value and coefficient of variation expressed in percentage (third row) of each counting method in microspore cultures at days 3 and 18
From: Comparison of six different methods to calculate cell densities
Method | Days 3 | Days 18 |
|---|---|---|
Neubauer | 371.8 (65.2) | 383.3 (79.3) |
359.4 (322.2–434.2) | 400.8 (328.6–437.2) | |
7.1/17.5% | 4.2/20.7% | |
FC unstained | 220.3 (90.1) | 255.2 (108.4) |
197.8 (173.7–247.6) | 255.2 (216.9–293.6) | |
44.9/40.9% | 36.2/42.5% | |
Flow cytometry + PI | 309.0 (60.2) | 332.0 (60.5) |
298.8 (273.3–324.6) | 320.6 (289.2–350.4) | |
22.8/19.5% | 17.0/18.2% | |
Flow cytometry + SSC | 305.4 (57.4) | 329.6 (60.5) |
296.5 (269.2–323.3) | 317.8 (285.9–350.2) | |
23.7/18.8% | 17.6/18.4% | |
Automated counter | 349.2 (50.3) | 335.5 (52.9) |
354.0 (299.0–385.5) | 349.5 (301.2–376.5) | |
12.7/14.4% | 16.0/15.9% | |
Field counting | 386.9 (81.5) | 336.4 (53.9) |
358.1 (315.8–467.9) | 341.4 (294.9–377.2) | |
3.3/21.1% | 15.9/16.0% |