Table 5 Normalized fluorescence levels (ΔRn values) obtained for SNP103 from the 20-fold dilutions using the three chemistries
From: Comparison of three PCR-based assays for SNP genotyping in plants
DNA input (ng) | TaqMan | KASP | rhAmp | |||
|---|---|---|---|---|---|---|
Allele1 | Allele2 | Allele1 | Allele2 | Allele1 | Allele2 | |
100 | 1.5 | 2.3 | 0.7 | 1.1 | 5.9 | 3.4 |
90 | 1.4 | 2.2 | 0.6 | 0.5 | 5.9 | 3.5 |
80 | 1.6 | 2.2 | 0.6 | 0.6 | 5.9 | 3.6 |
70 | 1.6 | 2.3 | 0.6 | 0.6 | 5.9 | 3.6 |
60 | 1.6 | 2.4 | 0.6 | 0.5 | 6.1 | 3.7 |
50 | 1.6 | 2.4 | 0.7 | 0.6 | 6.1 | 3.6 |
40 | 1.6 | 2.3 | 0.7 | 0.6 | 6.1 | 3.6 |
30 | 1.5 | 2.3 | 0.7 | 0.8 | 5.9 | 3.4 |
20 | 1.4 | 2.2 | 0.8 | 0.6 | 5.3 | 2.5 |
10 | 1.4 | 2.3 | 0.6 | 0.6 | 1.6 | 1.9 |
1 | 0.6 | 1.7 | 0.4 | 0.3 | 2.8 | 1.4 |
0.9 | 0.9 | 1.4 | 0.3 | 0.4 | 1.5 | 1.6 |
0.8 | 0.9 | 1.7 | 0.2 | 0.2 | 2.3 | 0.9 |
0.7 | 0.9 | 1.6 | 0.2 | 0.3 | 1.5 | 0.7 |
0.6 | 0.7 | 1.4 | 0.2 | 0.3 | 1.0 | 1.1 |
0.5 | 0.4 | 1.6 | 0.2 | 0.1 | 1.1 | 1.2 |
0.4 | 0.8 | 0.9 | 0.1 | 0.2 | 1.5 | 0.9 |
0.3 | 0.5 | 1.4 | 0.1 | 0.1 | 1.6 | 0.9 |
0.2 | 0.9 | 0.7 | 0.1 | 0.1 | 0.8 | 1.0 |
0.1 | 0.1 | 0.8 | 0.1 | 0.1 | 0.1 | 0.1 |
Water | 0.0 | 0.1 | 0.1 | 0.2 | 0.1 | 0.1 |