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Table 4 Average values for analysis of variance (ANOVA) showing the effect of TaqMan, KASP and rhAmp assays on quantitative metrics

From: Comparison of three PCR-based assays for SNP genotyping in plants

Assay

Call rate

No template control (NTC) location

Cluster to NTC distance

Cluster angle separation

Cluster spread

TaqMan

0.970a

0.85a

2.09b

43.96a

0.18b

KASP

0.976a

0.14b

1.72c

42.95a

0.16b

rhAmp

0.981a

0.86a

4.73a

41.73a

0.10a

  1. Different letters means significant difference at P < 0.05 according to Fisher’s Protected Least Significant Difference. No template control (NTC) location is the mean of four NTC reactions
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Plant Methods

ISSN: 1746-4811