Table 3 Analysis of variance (ANOVA) showing the effect of TaqMan, KASP and rhAmp assays on quantitative metrics
From: Comparison of three PCR-based assays for SNP genotyping in plants
Trait | Effect | df | Sum of squares | Mean squares | F | P |
|---|---|---|---|---|---|---|
Call rate | Assay | 2 | 0.031 | 0.016 | 2.5 | 0.090 |
Error | 96 | 0.612 | 0.006 | |||
Total | 98 | 0.643 | ||||
Cluster to NTC distance | Assay | 2 | 178.135 | 89.067 | 185.6 | < 0.001 |
Error | 96 | 46.078 | 0.480 | |||
Total | 98 | 224.213 | ||||
Cluster angle separation | Assay | 2 | 82.305 | 41.152 | 0.5 | 0.605 |
Error | 96 | 7813.594 | 81.392 | |||
Total | 98 | 7895.898 | ||||
Cluster spread | Assay | 2 | 0.683 | 0.342 | 38.8 | < 0.001 |
Error | 96 | 0.844 | 0.009 | |||
Total | 98 | 1.528 |