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Fig. 1 | Plant Methods

Fig. 1

From: Preparation and applications of guard cell protoplasts from the leaf epidermis of Solanum lycopersicum

Fig. 1

Guard cells at different stages of enzymatic digestion in Method L (ac) and Method S (dl). af Seven fully expanded leaves of the hydroponic plants selected for GCPs isolation (Additional file 1: Fig. S1). gi Seven fully expanded leaves of the substrate culture plants selected for GCPs isolation. jl Four fully expanded leaves of the hydroponic plants (younger plants compared with df) selected for GCPs isolation. a, d, h, k The status of GCs after 1.5, 0.5, 2 and 0.5 h of digestion in enzyme solution 1. b, e The status of GCs after 2 and 1 h of digestion in enzyme solution 2. c, f, i, l The status of GCs after 3.5, 2.5, 4.5 and 1.5 h of digestion in enzyme solution 2. Scale bars indicate 20 μm

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