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Fig. 1 | Plant Methods

Fig. 1

From: Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis

Fig. 1

Specificity of each S. viridis candidate reference gene primer pair. a Agarose gel analysis of RT-PCR generated amplicons for each of the eleven assessed candidate reference genes. M, marker (200 base pair gene ruler); 1, PP2A; 2, ASPR6; 3, PGM; 4, STK; 5, SEIPIN; 6, DUSP; 7, WNK1; 8, GRAS; 9, FBoxD; 10, CUL, and; 11, FPGS. b Melt curve analysis of the eleven candidate reference genes assessed across thirteen developmentally distinct tissues of S. viridis tested (including, internodes 4, 5 and 6; leaves 4, 5 and 6; inflorescence stem stages S1, S2 and S3, and; the four developmental zones of the expanding internode, internode 5) showed a single peak for each primer pair at a specific annealing temperature. The -(dF/dT) value represents the raw fluorescence (F) versus temperature (T) values

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