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Fig. 1 | Plant Methods

Fig. 1

From: Improved apple latent spherical virus-induced gene silencing in multiple soybean genotypes through direct inoculation of agro-infiltrated Nicotiana benthamiana extract

Fig. 1

Schematic representation of ALSV RNA1 (AR1), as well as three modified forms of ALSV RNA2 (AR2): AR2-GmPDS, AR2-NbPDS, and AR2-GmPDS-NbPDS, all of them flanked by a 35S promoter (P35S) and a NOS terminator (Tnos) to facilitate the synthesis of primary transcripts by RNA polymerase II of the host plant. Arrows correspond to primers used to amplify the corresponding regions of ALSV. GmPDS and NbPDS correspond to fragments derived from GmPDS1 and NbPDS coding sequences. A target gene can be inserted between MP and VP25 using XhoI and BamHI restriction enzymes

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