Table 2 Troubleshooting
Problem | Reason | Solution |
|---|---|---|
Poor germination and seedling growth | Used floaters Poor storage of seeds | Use sinkers Use desiccants during storage at 4 °C |
Lack of induction of hairy roots | Agrobacterium may lose virulence over the culturing process or become contaminated Seedling viability is too low Plants were not periodically watered | Use fresh − 80 °C glycerol stock Grow new seedlings Water plants regularly |