Skip to main content
Fig. 4 | Plant Methods

Fig. 4

From: Protocol: high-efficiency in-planta Agrobacterium-mediated transgenic hairy root induction of Camellia sinensis var. sinensis

Fig. 4

Validation of T-DNA insertion in hairy roots using PCR a amplification of rolC (Lane 1, 2) and aux1 (Lane 3, 4) from A4-wild type and A4-harboring pBI121 generated hairy roots; M, 1000 bp ladder; C, non-transformed roots; b amplification of uidA (GUS) gene from A4-harboring pBI121 generated hairy roots; M, 1000 bp ladder; C, transgenic hairy roots of A4-wild type; Lanes 1–4, transgenic hairy roots of A4 with uidA; c the relative transcript levels of the transgenes to the reference gene 18S rRNA showing their absence in the shoots of composite plants

Back to article page