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Fig. 5 | Plant Methods

Fig. 5

From: Isolation and manipulation of protoplasts from the unicellular green alga Penium margaritaceum

Fig. 5

Immunofluorescence (a) and transmission electron microscopy (TEM) (b–f) images of a Penium cell wall after treatment with 20 mM EDTA for 20 min and subsequent recovery. a JIM5 mAb immunolabeling of a cell recovered for 4 days. Note that homogalactan (HG) is produced at the isthmus (*) and is translocated outward toward the poles (arrows). b TEM of the cell wall of a recovering cell. New HG is produced at the isthmus zone (i) and is translocated outward toward the poles (white arrows). The polar zone contains the oldest wall of the cell (ii), and has no HG, which was removed by the treatment (white arrow). c INRA-RU1 mAb immunogold labeling of the isthmus zone of a cell recovered for 1 day shows the presence of gold particles at the isthmus (arrows). d JIM5 mAb immunogold labeling of the isthmus zone of a cell recovered for 1 day shows the start of a new cell wall being secreted, shown by the presence of few gold particles in the isthmus zone (arrows). e INRA-RU1 mAb immunogold labeling of the isthmus zone of a cell recovered for 4 days shows a dense labeling of the wall at the isthmus (arrows). f JIM5 mAb immunogold labeling of the isthmus zone of a cell recovered for 4 days shows the labeling of the wall at the isthmus (black arrow) and in the developing HG lattice (white arrows). IS, intracellular space. Scale bars: a = 15 µm; b = 2 µm; c, d = 400 nm; e, f = 500 nm

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