Fig. 5From: A novel rejuvenation approach to induce endohormones and improve rhizogenesis in mature Juglans treeGA3 immunolocalization in the stem bases of rejuvenated and mature soft shoots. a–f Transverse sections of rejuvenated soft shoots in walnut. a GA3was located in the sieve and companion cells of the stem after 1 day of induction. b By 3 days, GA3 signal began to appear in the cambium, but remained mainly distributed in sieve and companion cells (S&Cs). c By 4 days, as the cambium thickened, the GA3 signal became concentrated in the phloem and cambium, particularly along the ray cells. d By 5 days, some meristems cells had divided and the GA3 signals were distributed among meristems, S&Cs and ray cells. e By 7 days, GA3 signals were evident in the initial cell root primordia, S&Cs and the cambium. f GA3 signals were widely distributed in the root primordia by 9 days. g–i GA3 signals in the bases of mature soft shoots on day 1 (g), 5 (h), and 9 (i),GA3 was located mainly in the S&Cs of the phloem, by 9 days (i), signal were evident in part of cambium. The arrows indicate the GA3 signals. Arrowheads indicate the tissue structure. ca cambium, co cortex, m meristem, ph phloem, pi pith, rray cell, rp root primordia, rpic root primordia initial cells, S&Cs sieve and companion cells, sr sclerenchyma, xy xylem. Scale bars: 200 μm (e); 100 μm (b–d, f–i); and 50 μm (a)Back to article page