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Fig. 5 | Plant Methods

Fig. 5

From: A novel rejuvenation approach to induce endohormones and improve rhizogenesis in mature Juglans tree

Fig. 5

GA3 immunolocalization in the stem bases of rejuvenated and mature soft shoots. af Transverse sections of rejuvenated soft shoots in walnut. a GA3was located in the sieve and companion cells of the stem after 1 day of induction. b By 3 days, GA3 signal began to appear in the cambium, but remained mainly distributed in sieve and companion cells (S&Cs). c By 4 days, as the cambium thickened, the GA3 signal became concentrated in the phloem and cambium, particularly along the ray cells. d By 5 days, some meristems cells had divided and the GA3 signals were distributed among meristems, S&Cs and ray cells. e By 7 days, GA3 signals were evident in the initial cell root primordia, S&Cs and the cambium. f GA3 signals were widely distributed in the root primordia by 9 days. gi GA3 signals in the bases of mature soft shoots on day 1 (g), 5 (h), and 9 (i),GA3 was located mainly in the S&Cs of the phloem, by 9 days (i), signal were evident in part of cambium. The arrows indicate the GA3 signals. Arrowheads indicate the tissue structure. ca cambium, co cortex, m meristem, ph phloem, pi pith, rray cell, rp root primordia, rpic root primordia initial cells, S&Cs sieve and companion cells, sr sclerenchyma, xy xylem. Scale bars: 200 μm (e); 100 μm (bd, fi); and 50 μm (a)

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