Fig. 3
From: A novel rejuvenation approach to induce endohormones and improve rhizogenesis in mature Juglans tree
IAA immunolocalization in the stem bases of rejuvenated and mature soft shoots. a–f Transverse sections of rejuvenated soft shoots in walnut. a, b IAA is located in the stem 1–3 days after induction, but only in sieve cells and companion cells. c By 4 days, as the cambium thickens, IAA concentrates in this tissue. d By day 5, some meristem cells have divided and IAA is distributed in the meristems and ray cells. e IAA is present less in the initial cells of root primordia, part of cambium and S&Cs by 7 days. f The root primordia form by 9 days. IAA is distributed in the primordia, particularly in the tip regions. g–i IAA in the basal regions of mature soft shoots after 1 day (g), 5 days (h), and 9 days (i) of rooting induction. IAA signal is low at those times. The arrows indicate IAA signals. Arrowheads indicate the tissue structure. ca cambium, co cortex, m meristem, ph phloem, r ray cell, rp root primordia, rpic root primordia initial cells, S&Cs sieve and companion cells, sr sclerenchyma, xy xylem. Scale bars: 200 μm (e); 100 μm (b–d, f–i); 50 μm (a)